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Concentration profiles of solids suspended in a stirred ...

01.11.1986· The Peclet number Pe s is proportional to the solids concentration in the stirred tank under the present experimental conditions, while the parameter ratio Pe f /Pe s, which coincides with the ratio of the liquid flow velocity to the falling velocity of the solid particles, approaches a constant value as the concentration of solid particles is increased to 20vol.% or more.Cited by: 77

Optimum Solids Concentration in an Agitated Vessel ...

This study will propose optimum industrial agitator designs for systems handling high concentration slurries (up to 40% v/v) in the presence of gas.

Effect of agitation intensity on the biooxidation process ...

01.10.2012· The higher agitation intensity not only damaged the suspended cells but also prevented the adsorption of cells onto the mineral surface. The oxygen uptake rate (OUR), the Fe 2 + oxidation activity and the rus gene expression of A. ferrooxidans decreased with the agitation intensity during the biooxidation process, which might be responsible for the decrease of the biooxidation rates with the agitationCited by: 12

MIXING TIME IN UNBAFFLED STIRRED TANKS

A high-pass filter centered on the wavelength of fluoresced light (570nm) was placed in front of the camera in order to allow only this light to reach the CCD. The laser plane was focused in order to obtain a 0.5mm thin light sheet inside the vessel. The investigated vessel was an unbaffled cylindrical tank (T = 0.19m) stirred by a standard

Effect of aeration and agitation on the production of ...

08.04.2003· Methods and Results: The P. sinclairii was cultivated under various aeration and agitation conditions in a 5 l stirred‐tank bioreactor. The highest mycelial biomass (30·5 g l −1) and EPS production (11·5 g l −1) were obtained at a high aeration rate (3·5 v.v.m.) and at a high agitation speed (250 rev min −1). The apparent viscosities (6000–8000 cP) of fermentation broth increased rapidly towards the end of fermentations at high aeration and agitationCited by: 71

Continuous Stirred Tank Reactors - Visual Encyclopedia of ...

Continuous stirred-tank reactors are most commonly used in industrial processing, primarily in homogeneous liquid-phase flow reactions, where constant agitation is required. They may be used by themselves, in series, or in a battery. CSTRs are also used in

Alginate-chitosan core-shell microcapsule cultures of ...

Apr 17, 2021· The aforementioned results showed that at high agitation rate (100 rpm) in the stirred bioreactor, encapsulation matrix could not protect the hepatic cells from shear force damages. The results of cell behavior in the ACol/C microcapsules revealed that the matrix composition can affect cellular behavior at the same shear rate.

[PDF]MIXING TIME IN UNBAFFLED STIRRED TANKS

species whose concentration can be measured by a probe, e.g. conductivity [5, 6, 7]. ... 44 • Calculation of a final, steady state, background image in order to dump out image ... In Fig.4a, the values of tm and ts obtained at the various agitation speeds are reported as a function of Reynolds number.

Optimum Solids Concentration in an Agitated Vessel ...

Since the initial work of Drewer et al., 38 various researchers have used this approach to identify the optimum solids concentration in stirred 24, 34, 41,42 Considering that all these studies ...

(PDF) Effect of Operating Conditions on CSTR performance ...

The maximum conversion (99.5%) was obtained under optimum operating conditions of agitation rate (70 rpm) and reactants concentration (0.05 M) as evident from surface contours. View full-text Article

Amicon® Stirred Cells - Stirred Ultrafiltration Units

Amicon® stirred cells are ideal for finely separating macrosolutes (DNA, RNA, protein, etc.) in large-volume (3 mL-400 mL) samples. They are also reliable tools for membrane analysis, because they allow users to insert membranes of choice. Stirred cells concentrate solutions with up to 10% initial macrosolute concentration with high flow rates ...

(DOC) Effect of Pulse in Input Concentration to the ...

Systems connecting several CSTRs are used when the reaction is too slow. Multiple CSTRs can also be used when two immiscible liquids or viscous liquids are present and require a high agitation rate.(Hashsham, 2010) A CSTR can also function as a loop reactor when a heated, pressurized fluid is injected into the system to facilitate the stirring.

Cellule à agitation modèle 8050, 50 ml | UFSC05001

Cellule à agitation modèle 8050, 50 ml This Amicon stirred cell model provides high flow rates with solutions up to 10% macrosolute concentration. Capable of rapid concentration, or salt removal followed by concentration in the same unit. - Find MSDS or SDS, a

Evaluating Freeze-Thaw Processes in Biopharmaceutical ...

Jan 13, 2015· a high-concentration MAb-B drug product in glass vials and drug substance in plastic bottles a MAb-C drug substance in single-use bags. Here we show that understanding of a product’s robustness to freeze–thaw stress can be achieved using carefully designed scale-down models for freezing and thawing.

Modeling the Effect of L-Glutamine, Aeration and Agitation ...

Influence of substrate concentration for enhanced enzyme production was studied. Also effect of different aeration (0.5-2 vvm) and agitation rates (50 to 200 rpm) in a stirred tank reactor was examined.

Characterization of Minimum Impeller Speed for Suspension ...

The successful design and operation of Liquid-Solid (LS) and Gas-Liquid-Solid (GLS) stirred tank reactors requires an accurate determination of the level of solid suspension needed for the process at hand. A poor design of the stirred tank to achieve optimum conditions and maintain the system under these conditions during operation may cause significant drawbacks concerning product quality ...

Bioethanol Production from Fermentable Sugar Juice

Bioethanol production from renewable sources to be used in transportation is now an increasing demand worldwide due to continuous depletion of fossil fuels, economic and political crises, and growing concern on environmental safety. Mainly, three types of raw materials, that is, sugar juice, starchy crops, and lignocellulosic materials, are being used for this purpose.

[PDF]UNIVERSITI PUTRA MALAYSIA EFFECT OF FERMENTER ...

A\pergillus f/al'us Link strain 44-I and its relationship to kojic acid production was carried out using 2 L stirred tank fermenter. From the shake flask experiments, it was found that the addition of silicone based antifoam, even at very low concentration (0.00 I % v/v), reduced growth and

Alginate production by Pseudomonas mendocina in a stirred ...

Oct 26, 2006· Alginate production by P. mendocina is dependent on the carbon-to-nitrogen ratio (C:N) and high yields of this biopolymer were obtained in nitrogen-limited cultures. In previous assays carried out in shake flasks with 0.7–0.8 g l −1 of (NH 4) 2 SO 4 a polymer concentration of 12.3 g l −1 was obtained after 30 h fermentation from an initial glucose concentration of 20 g l −1.

Oxygen transfer and mixing in mechanically agitated ...

Airlift bioreactors and mechanically stirred tanks are widely used in bioprocessing [1,2]. Airlift bioreactors are useful with relatively less viscous fluids and when there is a need for gentle agitation [3] and low-cost oxygen transfer [4,5]. In comparison, conventional stirred fermenters have a broader range of applications but they perform poorly in highly viscous non-Newtonian media, have ...

Effect of aeration and agitation on the production of ...

8/4/2003· Methods and Results: The P. sinclairii was cultivated under various aeration and agitation conditions in a 5 l stirred‐tank bioreactor. The highest mycelial biomass (30·5 g l −1) and EPS production (11·5 g l −1) were obtained at a high aeration rate (3·5 v.v.m.) and at a high agitation speed (250 rev min −1). The apparent viscosities ...

Reactors - processdesign

22/2/2016· Stirred Tanks Stirred tanks were ... catalysis as long as the catalyst does not require continuous regeneration and the reaction mixture does not require high agitation (Towler, 2012). The amount of catalyst necessary can be found using the following equations: The ratio of the bed height (L) to the diameter (D) determines the distribution of reagents and the pressure drop across the bed. An ...

An effective method to release human islets from ...

Gentle agitation of embedded islets in high osmolality (500 mOsm/kg/H(2)O, 15 minutes) could release islets from surrounded exocrine tissue. An effective method to release human islets from surrounding acinar cells with agitation in high osmolality solution Transplant Proc. 2011 Nov;43(9):3161-6. doi: 10.1016/j.transproceed.2011.09.098. Authors M Shimoda 1 , T Itoh, K Sugimoto, M Takita, D ...

Designs | Free Full-Text | A Study of the Mixing ...

One of the main aims of agitation systems using stirred vessels is to maintain balanced quantities of substances in different phases based on concentration levels [].In cases where soluble solids are mixed, stirrers are used to increase interaction between the particles and avoid uneven accumulation at one point [].Flow streams in stirred vessels are known to be turbulent, chaotic and ...

High cell density cultivation and recombinant protein ...

30/5/2010· Hanson MA, Brorson KA, Moreira AR, Rao G: Comparisons of optically monitored small-scale stirred tank vessels to optically controlled disposable bag bioreactors. Microb Cell Fact. 2009, 8: 44- 10.1186/1475-2859-8-44. Article Google Scholar 6. Singh V: Disposable bioreactor for cell culture using wave-induced agitation. Cytotechnology. 1999, 30 ...

PRODUCTION OF FLAVONOID, AN ANTI-INFLAMMATORY AGENT

different agitation speed and volumetric air flow rate in stirred tank bioreactor of batch mode. 122 Table 4.10 Effect of volumetric air flow rate and agitation speed on oxygen uptake rate (OUR), volumetric oxygen transfer coefficients, k L a and oxygen transfer rate (OTR) in batch cultivation of T. lactinea in a stirred

Selection of human induced pluripotent stem cells lines ...

13/3/2020· Remarkably, FR202 showed high CM yields even when continuous agitation was applied (1.9 ± 0.03 × 10 6 cells/mL and purity of 83.2 ± 0.13 cTnT%; Table 1C). Collectively, out of 5 tested cell lines, the FR202 was selected for the cardiac bioprocess due to its high cardiac potential (Table 1 A), expansion potential on MCs (Table 1 B) and high CM production in continuously stirred MC spinner ...

CHAPTER 1 Intensified Fermentation Processes and Equipment ...

Assuming a glucose inlet concentration of 540 g kg −1, from an overall mass balance the biomass dry mass concentration follows as 39 kg m −3, which is relatively low. As a consequence, the relative biomass specific reaction rates will be high, e.g. q s = 4.0 kg glucose per kg biomass h or 0.54 mol s per mol x h (1.29) µ = 0.23 h −1 (1.30) q p = 1.4 kg BDO per kg biomass h or 0.38 mol p ...

Glucose limited feed strategy leads to increased ...

22/8/2018· This concentration is 12.5-fold higher than previously reported yields (Arens et al. 2014) and is as high as our results from batch shaking flasks (Halka and Wichmann 2018) which showed that a transfer to a scalable stirred tank reactor system is possible with similar results and shows more potential for the future. The combination of micro-aerobic fermentation conditions with the fed-batch ...